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Wiley, Protein Science, 6(4), p. 1233-1235, 1995

DOI: 10.1002/pro.5560040620

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Crystallization and preliminary X-ray investigation of lipoxygenase-3 from soybeans: Preliminary X-ray investigation of L-3

Journal article published in 1995 by Bernard Axelrod, Janusz Steczko, Wladek Minor ORCID, Vivian Stojanoff
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Soybean lipoxygenase-3 has been crystallized by the vapor diffusion method in 16-20% polyethylene glycol (average M(r), 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 degrees C, at a protein concentration of 8-15 mg/mL. The crystals, which diffract to 3-A spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b = 136.4, and c = 61.6 A and beta = 95.7 degrees. The calculated value of Matthews's constant, Vm = 2.48 A3/kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).