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Elsevier, Journal of Experimental Marine Biology and Ecology, 1(265), p. 101-115, 2001

DOI: 10.1016/s0022-0981(01)00327-6

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Combined sensitive analytical methods for cholecystokinin levels and tryptic activity in individual fish larvae

Journal article published in 2001 by Carlos R. Rojas-Garcia, Ivar Rönnestad ORCID, Bernd Ueberschär, Bernd Uebersch{̈a}r
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

A protocol for combined analysis of cholecystokinin (CCK) levels based on radioimmunoassay (RIA) and fluorescence tryptic enzyme activity (FTA) was developed in order to accomplish a sensitive analysis of individual bodies and gut segments of fish larvae. Methanol was used for CCK extraction. The gut of herring larvae contained 8.9±1.2 fmol CCK/mg dry weight and in the post-larval Atlantic halibut the CCK levels varied significantly (p