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American Society for Microbiology, Journal of Bacteriology, 4(184), p. 1003-1009, 2002

DOI: 10.1128/jb.184.4.1003-1009.2002

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Genetic Evidence that the uvsE Gene Product of Deinococcus radiodurans R1 Is a UV Damage Endonuclease

Journal article published in 2002 by Ashlee M. Earl ORCID, Sara K. Rankin, N. Oleana, Kwang-Pyo Kim, J. Bacteriol, Oleana N. Lamendola, John R. Battista
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1 + and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe . Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1 + strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.