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Elsevier, Molecular and Cellular Proteomics, 7(10), p. M000052-MCP200, 2011

DOI: 10.1074/mcp.m000052-mcp201

American Society for Biochemistry and Molecular Biology, Molecular and Cellular Proteomics, 7(10), p. M000052-MCP200-M000052-MCP200

DOI: 10.1074/mcp.m000052-mcp200

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Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR*

Journal article published in 2011 by Anton Steen, Tejas Gandhi, Elena Wiederhold, Rainer Breitling ORCID, Dirk Jan Slotboom
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Biochemical and biophysical characterization of CFTR (the cystic fibrosis transmembrane conductance regulator) is thwarted by difficulties to obtain sufficient quantities of correctly folded and functional protein. Here we have produced human CFTR in the prokaryotic expression host Lactococcus lactis. The full-length protein was detected in the membrane of the bacterium, but the yields were too low (