The study used a focal ischemia rat hindlimb model to investigate muscle activity changes during a 10-day body weight support (BWS) treadmill training program. The changes being studied included fatigue effects, EMG burst duration in the gait cycle, and the symmetry of muscle activation between affected and unaffected sides. Intramuscular EMG of medial gastrocnemius (MG) and tibialis anterior (TA) muscles in male Sprague Dawley rats at affected side (n=10) and unaffected side (n=10) were recorded during the treadmill running before a middle cerebral artery occlusion/reperfusion (MCAo/r) surgery and poststroke recovery stage. Behavioral test score and bodyweight were recorded at a daily basis after stroke. The mean power frequency (MPF) of the EMG, EMG burst duration in the gait cycle, and symmetry index between two sides were used for analysis. The drop rate of MPF of MG at the unaffected side increased (P