Elsevier, Biophysical Journal, 3(110), p. 194a-195a, 2016
DOI: 10.1016/j.bpj.2015.11.1084
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Since single-molecule FRET (smFRET) was demonstrated 20 years ago, tremendous progresses have been made in sample preparation and data analysis, allowing the study of increasingly complex systems and the extraction of precise structural information. However, technical improvements have been mostly confined to combining the technique with microfluidics or complementary techniques such as optical tweezers or atomic force microscopy.