The low solubility of As in mine soils limits its phytoavailability. This makes the extrapolation of data obtained under hydroponic conditions unrealistic because the concentration in nutrient solution frequently overexposes plants to this metalloid. This work evaluates whether As supply in hydroponics resembles, to some extent, the As phytoavailable fraction in soils and the implications for phytoremediation. Phytotoxicity of As, in terms of biomass production, chlorophyll levels, and As concentrations in plants, was estimated and compared in both soils and hydroponics. In order for hydroponic conditions to be compared to soil conditions, plant exposure levels were measured in both cultures. Hydroponic As concentration ranging from 2-8microM equated to the same plant organ concentrations from soils with 700-3000mgkg(-1). Total and extractable As fractions exceeded those values, but As concentrations in pore water were bellow them. According to our results (i) hydroponics should include doses in the range 0-10microM As to allow the extrapolation of the results to As-polluted soils, and (ii) phytoextraction of As in mining sites will be limited by low As phytoavailability.