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Nature Research, Nature Methods, 2(7), p. 119-122, 2010

DOI: 10.1038/nmeth.1416

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Parallel, tag-directed assembly of locally derived short sequence reads

Journal article published in 2010 by Joseph B. Hiatt, Rupali P. Patwardhan, Emily H. Turner, Choli Lee, Jay Shendure ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.