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Nature Research, Nature Methods, 8(6), p. 596-599, 2009

DOI: 10.1038/nmeth.1350

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Dereplication and De Novo Sequencing of Nonribosomal Peptides

Journal article published in 2009 by Julio Ng, Nuno Bandeira, Wei-Ting Liu, Majid Ghassemian, Thomas L. Simmons, William H. Gerwick, Roger Linington, Pieter C. Dorrestein, Pavel A. Pevzner
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP dereplication and sequencing. We employ multi-stage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also present an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but non-identical NRPs, substantially reducing dereplication efforts.