The purpose of this work was to study the effect of processing parameters (pressure and cycles) on the formation of microfluidized lemongrass oil-alginate nanoemulsions considering their average droplet size and size distribution, z-potential, viscosity and whiteness index. To confirm that nanoemulsions were in the nano-range, samples were also observed through transmission electron microscopy (TEM) and atomic force microscopy (AFM) techniques. Average droplet size, viscosity and whiteness index of nanoemulsions decreased by increasing the processing pressure and the cycles through the interaction chamber of the microfluidizer device. Nanoemulsions obtained at 150 MPa for 10 cycles exhibited a minimum average droplet size of 6 nm. Moreover, the droplet electrical charge of nanoemulsions ranged between 36.66 and 51.95 mV while it was 17.61 mV in the coarse emulsion. Furthermore, nanoemulsions obtained at 150 MPa for 3 times or more through the microfluidization system were almost transparent. Results obtained in the present study reveal that microfluidization is a potential technology to be used to produce nanoemulsions of essential oils. However, more information is needed about the influence of microfluidization conditions on the antimicrobial properties of essential oils dispersed in nano-sized emulsions. ; This study was supported by the Ministerio de Ciencia e Innovación (Spain) throughout the project AGL2009-11475. Laura Salvia-Trujillo thanks the Ministry of Science and Education (Spain) for the predoctoral grant.