Published in

American Society for Microbiology, Applied and Environmental Microbiology, 1(75), p. 252-256, 2009

DOI: 10.1128/aem.01630-08

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Selective extraction of bacterial DNA from the surfaces of macroalgae

Journal article published in 2008 by Catherine Burke, Staffan Kjelleberg, Torsten Thomas ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Preprint: archiving allowed
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Postprint: archiving allowed
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Published version: archiving restricted
Data provided by SHERPA/RoMEO

Abstract

ABSTRACT A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra . The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.