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Wiley, Angewandte Chemie International Edition, 29(53), p. 7531-7534, 2014

DOI: 10.1002/anie.201403890

Wiley, Angewandte Chemie, 29(126), p. 7661-7664, 2014

DOI: 10.1002/ange.201403890

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Ultrafluorogenic Coumarin-Tetrazine Probes for Real-Time Biological Imaging

Journal article published in 2014 by Labros G. Meimetis, Jonathan C. T. Carlson, Randy J. Giedt, Rainer H. Kohler, Ralph Weissleder ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We have developed a series of new ultrafluorogenic probes in the blue-green spectrum that display fluorescence enhancement exceeding 11,000-fold. These fluorogenic dyes integrate a coumarin fluorochrome with the bioorthogonal tetrazine-transcyclooctene (TCO) chemistry platform. By exploiting highly efficient through bond energy transfer (TBET), these probes exhibit the highest brightness enhancements reported thus far for any bioorthogonal fluorogenic dyes. No-wash, fluorogenic imaging of diverse targets including cell surface receptors in cancer cells, mitochondria, and the actin cytoskeleton is possible within seconds with minimal background signal and no appreciable non-specific binding, opening the possibility for in vivo sensing.