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Published in

Elsevier, Biophysical Journal, 9(93), p. 3314-3323, 2007

DOI: 10.1529/biophysj.107.111328

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Cell Force Microscopy on Elastic Layers of Finite Thickness

Journal article published in 2007 by Rudolf Merkel ORCID, Norbert Kirchgeßner, Claudia M. Cesa, Bernd Hoffmann
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm).