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Nature Research, Nature Communications, 1(5), 2014

DOI: 10.1038/ncomms5767

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Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

Journal article published in 2014 by Yao-Cheng Lin, Morgane Boone, Leander Meuris, Irma Lemmens, Nadine Van Roy, Arne Soete, Joke Reumers, Matthieu Moisse ORCID, Stéphane Plaisance, Radoje Drmanac, Jason Chen, Frank Speleman, Diether Lambrechts ORCID, Yves Van de Peer ORCID, Jan Tavernier and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The HEK293 human cell lineage is widely used in cell biology and biotechnology. We here use whole genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation (293T); suspension growth adaptation (293S) and cytotoxic lectin selection (293SG). Remarkably, we observe that copy number alteration detection could identify the genomic region that enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome data. We also establish that the genome structure composition is in steady state for most of these cell lines when standard cell culturing conditions are used. This resource enables novel and more informed studies with 293 cells, and we will distribute the sequenced cell lines to this effect. ; http://www.nature.com/naturecommunications ; hb2014