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Sociedade Brasileira de Microbiologia, Brazilian Journal of Microbiology, 2(45), p. 695-698

DOI: 10.1590/s1517-83822014000200044

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An improved purification procedure for Leishmania RNA virus (LRV)

Journal article published in 2014 by Marcos Michel de Souza, Marcos Michel de Souza, Livia Regina Manzine, Marcos Vinicius G. da Silva, Marcos Vinicius G. da Silva, Jefferson Bettini, Rodrigo Vilares Portugal, Angela Kaysel Cruz ORCID, Eurico Arruda, Otavio Henrique Thiemann
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.