American Society for Microbiology, Applied and Environmental Microbiology, 23(78), p. 8467-8469, 2012
DOI: 10.1128/aem.02288-12
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ABSTRACT We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B 12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.