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American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 12(56), p. 6410-6413, 2012

DOI: 10.1128/aac.01383-12

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Screening of Antifungal Azole Drugs and Agrochemicals with an Adapted alamarBlue-Based Assay Demonstrates Antibacterial Activity of Croconazole against Mycobacterium ulcerans

Journal article published in 2012 by Nicole Scherr, Katharina Röltgen ORCID, Matthias Witschel, Gerd Pluschke
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans . The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans .