Quantitative metabolism using AMS: Choosing a labeled precursor

Links, Jennifer; Palmblad, Magnus; Ognibene, Ted; Turteltaub, Ken; Bench, Graham

Published in

Elsevier, Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 7-8(268), p. 1309-1312

DOI: 10.1016/j.nimb.2009.10.160

Tools

Export citation

Search in Google Scholar

Quantitative metabolism using AMS: Choosing a labeled precursor

Journal article published in 2010 by Jennifer Links, Magnus Palmblad

, Ted Ognibene, Ken Turteltaub, Graham Bench

This paper is available in a repository.

Full text: Download

Preprint: archiving allowed

Upload

Postprint: archiving forbidden

Published version: archiving forbidden

Policy details

Data provided by

Abstract

Biological radioisotope studies suffer from a lack of sensitive measurement techniques and therefore traditionally require large amounts of labeled material to produce a measurable signal. Such quantities of materials are often significantly higher than naturally-occurring levels preventing these studies from replicating physiological conditions. AMS affords the sensitivity necessary to perform biological radioisotope studies with low levels of labeled material that preserve physiological conditions. The choice of labeled material can substantially affect the ease of interpretation and comprehensiveness of these studies. Here, the benefits and limitations of whole-cell labeling with (14)C-glucose and targeted pathway labeling with (14)C-nicotinic acid are discussed and compared.