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American Society for Microbiology, Journal of Bacteriology, 3(193), p. 670-678, 2011

DOI: 10.1128/jb.00750-10

American Society for Microbiology, Journal of Bacteriology, 6(194), p. 1641-1641, 2012

DOI: 10.1128/jb.00048-12

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African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa

Journal article published in 2010 by Stefan Berg ORCID, M. Carmen Garcia-Pelayo, Borna Müller, D. van Soolingen, Elena Hailu, Benon Asiimwe, Kristin Kremer, James Dale, M. Beatrice Boniotti, Sabrina Rodriguez ORCID, Markus Hilty, van Helden P., Leen Rigouts, Rebuma Firdessa, Adelina Machado and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS 6110 , in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.