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Mass spectrometry-based metabolomics of single yeast cells
Journal article published in 2013 by
Alfredo J. Ibáñez,
Stephan R. Fagerer,
Anna Mareike Schmidt,
Pawel L. Urban,
Konstantins Jefimovs,
Philipp Geiger,
Reinhard Dechant,
Matthias Heinemann 
,
Renato Zenobi
,
Renato Zenobi
Abstract
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (Delta PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.