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Nature Research, Nature Methods, 5(6), p. 339-342, 2009

DOI: 10.1038/nmeth.1324

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Super-Resolution Video Microscopy of Live Cells by Structured Illumination

Journal article published in 2009 by Peter Kner, Bryant B. Chhun, Eric R. Griffis ORCID, Lukman Winoto, Mats G. L. Gustafsson
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.