Dissemin is shutting down on January 1st, 2025

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Cell Press, Cell Reports, 4(4), p. 642-648, 2013

DOI: 10.1016/j.celrep.2013.07.020

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The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells.

Journal article published in 2013 by Göran Karlsson, Emma Rörby, Cristina Pina, Shamit Soneji, Kristian Reckzeh ORCID, Kenichi Miharada ORCID, Christine Karlsson, Yanping Guo, Cristina Fugazza, Rajeev Gupta, Jh H. Martens ORCID, Joost H A. Martens, Stefan Karlsson, Tariq Enver, Hg G. Stunnenberg
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.