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American Society for Microbiology, Journal of Clinical Microbiology, 4(52), p. 1217-1219, 2014

DOI: 10.1128/jcm.03106-13

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Rapid Identification of Bacteria and Candida Pathogens in Peritoneal Dialysis Effluent from Patients with Peritoneal Dialysis-Related Peritonitis by Use of Multilocus PCR Coupled with Electrospray Ionization Mass Spectrometry

Journal article published in 2014 by Yu-Tzu Chang, H.-C. Wang, Hsuan-Chen Wang, Ming-Cheng Wang, An-Bang Wu, Junne-Ming Sung ORCID, H. Sunny Sun, Ih-Jen Su, Wei-Chih Kan, Chih-Chiang Chien, Jyh-Chang Hwang, Hsien-Yi Wang, H.-Y. Wang, Chin-Chung Tseng ORCID, Chi-Jung Wu
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.