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Springer Verlag, Protoplasma, 2(251), p. 449-458

DOI: 10.1007/s00709-013-0583-y

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Cryo-soft X-ray tomography: a journey into the world of the native-state cell

Journal article published in 2013 by Raffaella Carzaniga, Marie-Charlotte Domart, Lucy M. Collinson ORCID, Elizabeth Duke
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

One of the ultimate aims of imaging in biology is to achieve molecular localisation in the context of the structure of cells in their native state. Here, we review the current state of the art in cryo-soft X-ray tomography (cryo-SXT), which is the only imaging modality that can provide nanoscale 3D information from cryo-preserved, unstained, whole cells thicker than 1 μm. Correlative cryo-fluorescence and cryo-SXT adds functional information to structure, enabling studies of cellular events that cannot be captured using light, electron or X-ray microscopes alone.