Published in
Wiley Open Access, Molecular Systems Biology, 1(8), p. 581, 2012
DOI: 10.1038/msb.2012.13
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- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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Prediction and identification of sequences coding for orphan enzymes using genomic and metagenomic neighbours
Journal article published in 2012 by
Takuji Yamada,
Alison S. Waller,
Jeroen Raes 
,
Aleksej Zelezniak,
Nadia Perchat,
Alain Perret,
Marcel Salanoubat,
Kiran R. Patil,
Jean Weissenbach,
Peer Bork
,
Aleksej Zelezniak,
Nadia Perchat,
Alain Perret,
Marcel Salanoubat,
Kiran R. Patil,
Jean Weissenbach,
Peer Bork
This paper is made freely available by the publisher.
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Abstract
Many characterized metabolic enzymes currently lack associated gene and protein sequences. Here, pathway and genomic neighbour data are used to assign genes to these ‘orphan enzymes,' and the predictions are validated with experimental assays and genome-scale metabolic modelling.