Using eye samples of nine 9-week-old experimentally West Nile virus (WNV)–infected red-legged partridges (Alectoris rufa), time course of lesions and WNV antigen appearance in ocular structures were examined. In addition, eye samples of 6 red-legged partridges and 3 common pheasants (Phasianus colchicus) naturally infected with Bagaza virus (BAGV) were used to study lesions and flavivirus antigen distribution in relation to apparent blindness in the former. The rapid onset of microscopic lesions and early presence of viral antigen in the eye of experimentally WNV-infected partridges, prior to the central nervous system involvement, suggested hematogenous spread of the virus into the eye. BAGV-infected partridges had a more pronunced inflammatory reaction and more widespread flavivirus antigen distribution in the retina compared with pheasants and experimentally fatally WNV-infected partridges. Our results suggest that flavivirus replication and development of lesions in ocular structures of gallinaceous game birds vary with the specific virus and host species involved. ; This study was supported by grants AG2008-02504GAN and SAF-2008-04232 funded by the Spanish Ministry for Science and Innovation, grants FAU2008-00006 and RTA2011-00036 from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), and The Network of Animal Disease Infectiology and Research Facilities, NADIR-EU-228394, funded by the EU. ; Peer Reviewed