Published in

American Chemical Society, Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 8(117), p. 2267-2272, 2013

DOI: 10.1021/jp3096715

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Sorting Short Fragments of Single-Stranded DNA with an Evolving Electric Double Layer

Journal article published in 2013 by Jiamin Wu ORCID, Shuang-Liang Zhao, Lizeng Gao, Jianzhong Wu, Di Gao
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We demonstrate a new procedure for separation of single-stranded DNA (ssDNA) fragments that are anchored to the surface of a gold electrode by end hybridization. The new separation procedure takes the advantage of the strong yet evolving non-uniform electric field near the gold surface in contact with a buffer solution gradually being diluted with deionized water. Separation of short ssDNA fragments is demonstrated by monitoring the DNA at the gold surface with in situ fluorescence measurement. The experimental results can be rationalized with a simple theoretical model of electric double layer that relates the strength of the surface pulling force to the ionic concentration of the changing buffer solution.