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BioMed Central, Respiratory Research, 1(11), 2010

DOI: 10.1186/1465-9921-11-76

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Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients

Journal article published in 2010 by Richard A. Urbanowicz ORCID, Jonathan R. Lamb, Ian Todd, Jonathan M. Corne, Lucy C. Fairclough
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract Background We have previously shown that NK (CD56 + CD3 - ) and NKT-like (CD56 + CD3 + ) cells are reduced in both numbers and cytotoxicity in peripheral blood. The aim of the present study was to investigate their numbers and function within induced sputum. Methods Induced sputum cell numbers and intracellular granzyme B and perforin were analysed by flow cytometry. Immunomagnetically selected CD56 + cells (NK and NKT-like cells) were used in an LDH release assay to determine cytotoxicity. Results The proportion of NK cells and NKT-like cells in smokers with COPD (COPD subjects) was significantly higher (12.7% and 3%, respectively) than in healthy smokers (smokers) (5.7%, p