Dissemin is shutting down on January 1st, 2025

Published in

Elsevier, Journal of Lipid Research, 12(52), p. 2341-2351, 2011

DOI: 10.1194/jlr.d018937

Links

Tools

Export citation

Search in Google Scholar

A biochemical fluorometric method for assessing the oxidative properties of HDL

Journal article published in 2011 by Theodoros Kelesidis, Judith S. Currier, Diana Huynh, David Meriwether ORCID, Christina Charles-Schoeman, Srinivasa T. Reddy, Alan M. Fogelman, Mohamad Navab, Otto O. Yang
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Most current assays of HDL functional properties are cell-based. We have developed a fluorometric biochemical assay based on the oxidation of dihydrorhodamine 123 (DHR) by HDL. This cell-free assay assesses the intrinsic ability of HDL to be oxidized by measuring increasing fluorescence due to DHR oxidation over time. The assay distinguishes the oxidative potential of HDL taken from different persons, and the results are reproducible. Direct comparison of this measurement correlated well with results obtained using a validated cell-based assay (r2 = 0.62, P