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American Society for Microbiology, Journal of Clinical Microbiology, 1(42), p. 408-411, 2004

DOI: 10.1128/jcm.42.1.408-411.2004

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Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

Journal article published in 2004 by Petra Wolffs ORCID, Halfdan Grage, Oskar Hagberg, Peter Rådström
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.