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Nature Research, Nature Methods, 6(11), p. 625-628, 2014

DOI: 10.1038/nmeth.2925

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Rapid adaptive optical recovery of optimal resolution over large volumes

Journal article published in 2014 by Kai Wang, Daniel E. Milkie, Ankur Saxena, Peter Engerer, Thomas Misgeld, Marianne E. Bronner ORCID, Jeff Mumm ORCID, Eric Betzig
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.