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- [www.ncbi.nlm.nih.gov] | PDF
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- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
Tools
Semi-automated microseeding of nanolitre crystallization experiments
Journal article published in 2007 by
Thomas S. Walter,
Erika J. Mancini 
,
Jan Kadlec,
Stephen C. Graham,
René Assenberg,
Jingshan Ren,
Sarah Sainsbury,
Raymond J. Owens,
David I. Stuart,
Jonathan M. Grimes,
Karl Harlos
,
Jan Kadlec,
Stephen C. Graham,
René Assenberg,
Jingshan Ren,
Sarah Sainsbury,
Raymond J. Owens,
David I. Stuart,
Jonathan M. Grimes,
Karl Harlos
Abstract
A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.