Microlens-ended fibers, which have found tremendous interest in the recent past, find potential biomedical applications, in particular, in endoscopic imaging. The work presented in this paper focuses on the stand-alone microlenses along with custom-fabricated specialty optical fiber, such as imaging fiber, for probe imaging applications. Stand-alone self-aligned microlenses have been fabricated employing microcompression molding and then attached at the end facet of imaging fiber. A detailed characterization of the fabricated microlens is carried and it demonstrates appropriate focusing ability, high fluorescence collection efficiency and imaging ability for biomedical applications. The surface roughness of the microlens is found to be 25 nm with a minimum spot size of 38 μm. The probe imaging system is found to be able to image the fluorescence microspheres of 10 μm size. The collection efficiency of the fiber probe with lens found to be enhanced by three times approximately.