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American Society for Microbiology, Journal of Virology, 9(77), p. 5487-5492, 2003

DOI: 10.1128/jvi.77.9.5487-5492.2003

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Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons

Journal article published in 2003 by Rainer Gosert, Denise Egger, Volker Lohmann ORCID, Ralf Bartenschlager, Hubert E. Blum, Kurt Bienz, Darius Moradpour
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5′-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.