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Public Library of Science, PLoS ONE, 10(8), p. e76751, 2013

DOI: 10.1371/journal.pone.0076751

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Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties

Journal article published in 2013 by William A. Braff, Dana Willner, Philip Hugenholtz ORCID, Korneel Rabaey, Cullen R. Buie
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.