This work shows a study on the micro milling of Ti-6Al-4V Titanium alloy where the effect of tool wear on the surface finish of the machined part was analyzed. New and worn micro cutters were applied to produce grooves with 0.5 mm of width and 0.025 mm of heights. The surface roughness was measured in the Ra and Rz values and the results showed that the surface roughness was not influenced by the tool’s condition. However, when new tools were used the increase of cutting speed generated an improving of the surface roughness and the same effect occurred with the decrease of feed rate.