Published in

Current Protocols in Molecular Biology

DOI: 10.1002/0471142727.mb1310bs93

Links

Tools

Export citation

Search in Google Scholar

Conditional Depletion of Nuclear Proteins by the Anchor Away System

Journal article published in 2011 by Xiaochun Fan, Joseph V. Geisberg, Koon Ho Wong ORCID, Yi Jin
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Red circle
Preprint: archiving forbidden
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus.