In the wake of letters containing anthrax spores terrifying the USA and other letters containing unidentified white powders circulating all over the world, the threat of bioterrorism attracts the attention of the general public as well as scientist. Therefore, it is urgent to develop rapid, sensitive, and high-throughput diagnostic methods able to counter attacks of bioterrorism by elucidating the suitable actions that should be implemented to prevent serious epidemic diseases. Numerous such methods are in development but Nucleic Acid Detection is the standard employed for identifying most biological agents that are used in bioterrorism. This method is based on PCR assays via the classical techniques of amplification and fluorescent detection. On the other hand, electrochemical biosensors are promising platforms that could achieve rapid highly sensitive and selective onsite detection of such agents. This chapter will present the recent developments in electrochemical biosensors for preparing DNA detection platforms that could be used to prevent attacks of bioterrorism.