A variety of diseases of wheat (Triticum aestivum L.) occurs every year in the U.S. leading to significant grain yield losses. Stagonospora nodorum blotch (SNB), fusarium head blight (FHB) and stem rust (SR) are caused by the fungi Stagonospora nodorum, Fusarium graminearum and Puccinia graminis, respectively. These diseases penalize both grain yield and quality. Three resistance factors, Qsng.sfr-3BS, Fhb1 and Sr2 conferring resistance, respectively, to SNB, FHB and SR, each from a unique donor wheat parent line, have been mapped to chromosome 3BS of wheat and are believed to be closely linked. Based on previously published analyses, Sr2 is on the distal end, Fhb1 is on the proximal end and Qsng.sfr-3BS is in the middle of Sr2 and Fhb1 in the 3BS wheat genome. Thus, the objectives of this project are to determine the gene order of Qsng.sfr-3BS, Fhb1 and Sr2, in a linkage block on chromosome 3BS and combining them in coupling. The linkage relationships were determined through analysis of a three-way cross between parental lines Arina, Alsen and Ocoroni86, containing the resistance genes Qsng.sfr-3BS, Fhb1 and Sr2, respectively. A total of 1,600 F2 plants was screened, along with the parental lines, using KASPar genotyping technology via single-nucleotide polymorphism markers to identify the recombinant progeny. Phenotypic screening for SNB was performed on the entire F2 population. Knowing the positional order of these resistance genes will enable the development of a wheat line with three genes in coupling to provide durable and broad-spectrum resistance against three major diseases of wheat.