Hymenaea coubaril L. seeds are widely used by the population for the treatment of several ailments. Despite its popular use, no other phytochemical studies had been carried out about "Jatobá" seeds. For this reason, this is the first study on the matter and it was carried out with the purpose of finding and identifying the molecules that constitute this seed. This work presented the isolation and elucidation of two new biscoumarins from H. coubaril L. seeds. The new compounds: hymenain 7-O-β-glucopyranosyl-(1′′′ → 2′′)-O-α-apiofuranosyl-(1′′′′ → 2′′′)-O-α-galactopyranoside (1) and hymenain 7-O-β-glucopyranosyl-(1′′′ → 2′′)-O-α-apiofuranoside (2). Their structures were elucidated by extensive 1D and 2D NMR experiments, IR and electrospray high resolution mass spectrometry (ESI-HRMS). © 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.