Petiveria alliacea L. (anamu) is a plant largely used in traditional medicine. It has a peculiar chemical composition determined by the abundance of sulphur compounds. The presence of these compounds in the plant, besides conferring it antimicrobial properties, implies the possibility of its use in the phytoremediation. The previous statement indicates that the medical prescription of this species requires the imminent fulfillment of previous tests of the metals content in its tissues, which might conduct, in levels higher to the ones permissible, to the deterioration of the human health. In the present research it was determined by Atomic Emission Spectrometry with Inductively Coupled Plasma the concentrations of 12 metals in a sample of Petiveria alliacea L. leaves, used in the elaboration of total extracts with in vitro antimicrobial activity against pathogens for human species. The quantification of total phenols in the extracts were performed by colorimetric method of Folin-Ciocalteu. The quantification of total flavonoids in the extracts were carried out by the colorimetric method based on the reaction with 2,5 % aluminum chloride in methanol. The values of metal concentrations were lower than maximum levels permissible in medicine herbs suggested by the World Health Organization. The concentrations of total phenols in the extracts ranged from 0,06 to 41,50 mg/mL and the total flavonoids were found in concentrations between 0,460 and 21,80 mg/mL.