Measuring system for recording driver reaction time was built, where computer sound card was used as a data acquisition system. An experiment in virtual environment was performed to evaluate proposed measuring system. Measured values of reaction time were similar to reported values of studies in real-world on public roads. In addition, significant changes of recorded physiological parameters during experiment indicate that subjects responded similar to the real-world situations. From our results we can conclude that the built low-cost measuring system was sufficient to detect response of a driver to a hazardous situation and is ready to be tested in real-world situations. Copyright