A stable enzyme-free glucose sensor was fabricated via a facile in situ hydrothermal route by the formation of CuFe2O4 nanoparticles into the graphene oxide sheets. The contents of graphene oxide in composites were varied from 10 to 30 wt%. The morphology of formed CuFe2O4/rGO nanocomposite was found by TEM analysis. The electrocatalytic activity of different CuFe2O4/rGO samples towards glucose oxidation was studied by employing cyclic voltammetry and chronoamperometry techniques. CuFe2O4/rGO(30 wt%) sample showed the highest oxidation current. An excellent sensitivity value of 1824.22 μA mM−1 cm−2 with a detection limit of 1.0 μM was gained by CuFe2O4/rGO(30 wt%). The sensor also exhibits good reproducibility and long term stability as well as high selectivity with insignificant interference from ascorbic acid and uric acid.