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American Society for Microbiology, Applied and Environmental Microbiology, 17(76), p. 5990-5994, 2010

DOI: 10.1128/aem.00701-10

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Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters

Journal article published in 2010 by Thomas Drepper, Robert Huber, Achim Heck ORCID, Franco Circolone, Anne-Kathrin Hillmer, Jochen Büchs, Karl-Erich Jaeger
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these conditions.