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- [www.ncbi.nlm.nih.gov] | PDF
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- [www.ncbi.nlm.nih.gov] | PDF
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A mass spectrometry–based hybrid method for structural modeling of protein complexes
Journal article published in 2014 by
Argyris Politis,
Florian Stengel,
Zoe Hall 
,
Helena Hernández,
Alexander Leitner,
Thomas Walzthoeni,
Carol V. Robinson,
Ruedi Aebersold
,
Helena Hernández,
Alexander Leitner,
Thomas Walzthoeni,
Carol V. Robinson,
Ruedi Aebersold
Abstract
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.