The goal of this study is to identify and compare palatal rugae patterns in males and females of average age of 35 in the Bosnia and Herzegovina population sample (Sarajevo Canton), as an additional method for sex differentiation in various situations. The research did not determine any statistically significant difference in the total number of palatal rugae in different sexes, and that is in line with previous research. However, in case of the number of secondary palatal rugae with probability of equal average is slightly higher than the tolerance of 5%, the difference being 7.6% may indicate that secondary palatal rugae are more common in women. However, LRA is still more successful in classifying males, 69% of them, while for women the success rate is significantly lower with only 41%. In total, 55% of subjects were correctly classified. It may be concluded that using LRA in palatal rugae could be used as an additional sex differentiation method for BiH population.