Sorting of GFP positive cells by flow cytometry. Representative plots of the cell sorting used in Figure 6. Cell events are denoted by dots. Increasingly "hot" colours represent increasing density of cell events. In each panel, the cells selected for further sorting are outlined by a polygon (termed a gate), and the inlaid number indicates the percentage of cells at that stage within the gate. A. Cells were gated based on forward and side scatter to eliminate debris. B. Doublet discrimination was carried out to ensure only single cells were sorted. C. Live/dead cells were discriminated with To-Pro-3 staining, detected using a 670/30 filter. D. GFP fluorescence was detected using a 530/30 filter.