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Nature Research, Scientific Reports, 1(6), 2016

DOI: 10.1038/srep22354

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Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography

Journal article published in 2016 by Jose Javier Conesa ORCID, Joaquín Otón ORCID, Michele Chiappi, Jose María Carazo ORCID, Eva Pereiro, Francisco Javier Chichón, José L. Carrascosa
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

AbstractWe used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm3 sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.