Elsevier, Developmental Cell, 3(16), p. 482, 2009
DOI: 10.1016/j.devcel.2009.02.014
Elsevier, Developmental Cell, 5(15), p. 781-791, 2008
DOI: 10.1016/j.devcel.2008.09.007
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Kidney development is based on differential cell type specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genomewide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescent activated cell sorting, followed by microarray profiling. The resulting data agrees with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of novel growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.