Fig. S2 Immunoblotting profile of Sj23HD recognition by serum IgG of 10 individual mice infected with S. japonicum at different times. For all blots, lane M, protein molecular weight markers; lane 1, no protein band was recognized by pooled sera of five mice before infection; lanes 2-11, sera of 10 individual mice on the indicated days post-infection. Black arrows indicate the position of IgG reactive Sj23HD protein band (33.5 kDa). The Sj23 HD protein was recognized by serum IgG of: (A) one mouse (lane 3) at day 7 post-infection; (B) one mouse (lane 8) at day 10 post-infection; (C) three mice (lanes 3, 4, 8) at day 14 post-infection; (D) five mice (lanes 4-8) at day 18 post-infection; (E) eight mice (lanes 2-9) at day 21 post-infection; (F) and all 10 mice (lanes 2-11) at day 28 post-infection.